ELISA Kit for Corin (CRN)

ATC2; CRN; Lrp4; TMPRSS10; Atrial Natriuretic Peptide-Converting Enzyme, Serine Protease; Heart-specific serine proteinase ATC2; Pro-ANP-converting enzyme

  • ELISA Kit for Corin (CRN) Packages (Simulation)
  • ELISA Kit for Corin (CRN) Packages (Simulation)
  • ELISA Kit for Corin (CRN) Results demonstration
  • CEG316Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Corin (CRN)

This assay has high sensitivity and excellent specificity for detection of Corin (CRN).
No significant cross-reactivity or interference between Corin (CRN) and analogues was observed.

Recovery of the ELISA Kit for Corin (CRN)

Matrices listed below were spiked with certain level of recombinant Corin (CRN) and the recovery rates were calculated by comparing the measured value to the expected amount of Corin (CRN) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-101 97
EDTA plasma(n=5) 88-95 92
heparin plasma(n=5) 92-101 96

Precision of the ELISA Kit for Corin (CRN)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corin (CRN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corin (CRN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Corin (CRN)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Corin (CRN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-99% 86-95% 94-102% 96-104%
EDTA plasma(n=5) 90-102% 79-101% 84-91% 98-105%
heparin plasma(n=5) 80-101% 78-96% 97-104% 79-96%

Stability of the ELISA Kit for Corin (CRN)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Corin (CRN)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle of the ELISA Kit for Corin (CRN)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Corin (CRN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Corin (CRN) and unlabeled Corin (CRN) (Standards or samples) with the pre-coated antibody specific to Corin (CRN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Corin (CRN) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Corin (CRN) in the sample.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPG316Hu02 Recombinant Corin (CRN) Positive Control; Immunogen; SDS-PAGE; WB.
RPG316Hu01 Recombinant Corin (CRN) Positive Control; Immunogen; SDS-PAGE; WB.
RPG316Hu04 Recombinant Corin (CRN) Positive Control; Immunogen; SDS-PAGE; WB.
RPG316Hu03 Recombinant Corin (CRN) Positive Control; Immunogen; SDS-PAGE; WB.
PAG316Hu01 Polyclonal Antibody to Corin (CRN) WB; IHC; ICC; IP.
PAG316Hu04 Polyclonal Antibody to Corin (CRN) WB; IHC; ICC; IP.
PAG316Hu02 Polyclonal Antibody to Corin (CRN) WB; IHC; ICC; IP.
PAG316Hu03 Polyclonal Antibody to Corin (CRN) WB; IHC; ICC; IP.
MAG316Hu21 Monoclonal Antibody to Corin (CRN) WB; IHC; ICC; IP.
SEG316Hu ELISA Kit for Corin (CRN) Enzyme-linked immunosorbent assay for Antigen Detection.
CEG316Hu ELISA Kit for Corin (CRN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMG316Hu Magnetic Luminex Assay Kit for Corin (CRN) ,etc. Magnetic Luminex Assay for Antigen Detection.
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