ELISA Kit DIY Materials for Vitamin D3 (VD3)
Cholecalciferol; Toxiferol; Activated 7-Dehydrocholesterol
- Product No.KSA920Ge11
- Organism SpeciesPan-species (General) Same name, Different species.
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma and other biological fluids
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range4.94-400ng/mL
- Applicable Sensitivity1.85ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Download n/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
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FOB
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the ELISA Kit DIY Materials for Vitamin D3 (VD3)
The Abs in the kit have high sensitivity and excellent specificity for detection of Vitamin D3 (VD3). No significant cross-reactivity or interference between Vitamin D3 (VD3) and analogues was observed.
USAGE of the ELISA Kit DIY Materials for Vitamin D3 (VD3)
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE of the ELISA Kit DIY Materials for Vitamin D3 (VD3)
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
SUPPORT PACK of the ELISA Kit DIY Materials for Vitamin D3 (VD3)
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