ELISA Kit DIY Materials for Tight Junction Associated Protein 1, Peripheral (TJAP1)
PILT; TJP4; Tight Junction Protein 4; Protein incorporated later into tight junctions
- Product No.UKSF168Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range78-5,000pg/mL
- Applicable Sensitivity34pg/mL
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
- Download n/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the ELISA Kit DIY Materials for Tight Junction Associated Protein 1, Peripheral (TJAP1)
The Abs in the kit have high sensitivity and excellent specificity for detection of Tight Junction Associated Protein 1, Peripheral (TJAP1). No significant cross-reactivity or interference between Tight Junction Associated Protein 1, Peripheral (TJAP1) and analogues was observed.
USAGE of the ELISA Kit DIY Materials for Tight Junction Associated Protein 1, Peripheral (TJAP1)
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE of the ELISA Kit DIY Materials for Tight Junction Associated Protein 1, Peripheral (TJAP1)
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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