ELISA Kit DIY Materials for Endothelin 1 (EDN1)
ET1; PPET1; Preproendothelin-1; Big endothelin-1
- Product No.UKSA482Mu11
 - Organism SpeciesMus musculus (Mouse) Same name, Different species.
 - Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
 - Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
 - Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
 - Detectable Range6.17-500pg/mL
 - Applicable Sensitivity2.43pg/mL
 - ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
 - Download n/a
 - UOM 96T*596T*10 96T*20 96T*50 96T*100
 - 
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SPECIFITY of the ELISA Kit DIY Materials for Endothelin 1 (EDN1)
The Abs in the kit have high sensitivity and excellent specificity for detection of Endothelin 1 (EDN1). No significant cross-reactivity or interference between Endothelin 1 (EDN1) and analogues was observed.
USAGE of the ELISA Kit DIY Materials for Endothelin 1 (EDN1)
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE of the ELISA Kit DIY Materials for Endothelin 1 (EDN1)
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) | 
| URPA482Mu02 | Recombinant Endothelin 1 (EDN1) | Positive Control; Immunogen; SDS-PAGE; WB. | 
| URPA482Mu01 | Recombinant Endothelin 1 (EDN1) | Positive Control; Immunogen; SDS-PAGE; WB. | 
| UCPA482Mu11 | BSA Conjugated Endothelin 1 (EDN1) | Immunogen; SDS-PAGE; WB. | 
| UCPA482Mu21 | OVA Conjugated Endothelin 1 (EDN1) | Immunogen; SDS-PAGE; WB. | 
| UPAA482Mu08 | Polyclonal Antibody to Endothelin 1 (EDN1) | WB; IHC; ICC; IP. | 
| UPAA482Mu02 | Polyclonal Antibody to Endothelin 1 (EDN1) | WB; IHC; ICC; IP. | 
| UPAA482Mu01 | Polyclonal Antibody to Endothelin 1 (EDN1) | WB; IHC | 
| UCEA482Mu | ELISA Kit for Endothelin 1 (EDN1) | Enzyme-linked immunosorbent assay for Antigen Detection. | 
| ULMA482Mu | Multiplex Assay Kit for Endothelin 1 (EDN1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. | 
| UKSA482Mu11 | ELISA Kit DIY Materials for Endothelin 1 (EDN1) | Main materials for "Do It (ELISA Kit) Yourself". | 
