CLIA Kit for Zymogen Granule Protein 16 Homolog B (ZG16B)

JCLN2; Jacalin-Like Lectin Domain Containing 2

  • CLIA Kit for Zymogen Granule Protein 16 Homolog B (ZG16B) Packages (Simulation)
  • CLIA Kit for Zymogen Granule Protein 16 Homolog B (ZG16B) Packages (Simulation)
  • CLIA Kit for Zymogen Granule Protein 16 Homolog B (ZG16B) Results demonstration
  • SCS158Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Zymogen Granule Protein 16 Homolog B (ZG16B)

This assay has high sensitivity and excellent specificity for detection of Zymogen Granule Protein 16 Homolog B (ZG16B).
No significant cross-reactivity or interference between Zymogen Granule Protein 16 Homolog B (ZG16B) and analogues was observed.

Recovery of the CLIA Kit for Zymogen Granule Protein 16 Homolog B (ZG16B)

Matrices listed below were spiked with certain level of recombinant Zymogen Granule Protein 16 Homolog B (ZG16B) and the recovery rates were calculated by comparing the measured value to the expected amount of Zymogen Granule Protein 16 Homolog B (ZG16B) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-97 86
EDTA plasma(n=5) 95-102 98
heparin plasma(n=5) 98-105 102

Precision of the CLIA Kit for Zymogen Granule Protein 16 Homolog B (ZG16B)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Zymogen Granule Protein 16 Homolog B (ZG16B) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Zymogen Granule Protein 16 Homolog B (ZG16B) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Zymogen Granule Protein 16 Homolog B (ZG16B)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Zymogen Granule Protein 16 Homolog B (ZG16B) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-90% 95-105% 87-105% 86-103%
EDTA plasma(n=5) 97-105% 97-104% 88-95% 78-94%
heparin plasma(n=5) 90-97% 89-96% 94-105% 94-103%

Stability of the CLIA Kit for Zymogen Granule Protein 16 Homolog B (ZG16B)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Zymogen Granule Protein 16 Homolog B (ZG16B)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Zymogen Granule Protein 16 Homolog B (ZG16B)

The microplate provided in this kit has been pre-coated with an antibody specific to Zymogen Granule Protein 16 Homolog B (ZG16B). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Zymogen Granule Protein 16 Homolog B (ZG16B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Zymogen Granule Protein 16 Homolog B (ZG16B) level in the sample or standard.;

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPS158Hu01 Recombinant Zymogen Granule Protein 16 Homolog B (ZG16B) Positive Control; Immunogen; SDS-PAGE; WB.
RPS158Hu02 Recombinant Zymogen Granule Protein 16 Homolog B (ZG16B) Positive Control; Immunogen; SDS-PAGE; WB.
PAS158Hu01 Polyclonal Antibody to Zymogen Granule Protein 16 Homolog B (ZG16B) WB; ICC/IF;FCM
MAS158Hu21 Monoclonal Antibody to Zymogen Granule Protein 16 Homolog B (ZG16B) WB; IHC; ICC; IP.
SES158Hu ELISA Kit for Zymogen Granule Protein 16 Homolog B (ZG16B) Enzyme-linked immunosorbent assay for Antigen Detection.
SCS158Hu CLIA Kit for Zymogen Granule Protein 16 Homolog B (ZG16B) Chemiluminescent immunoassay for Antigen Detection.
LMS158Hu Multiplex Assay Kit for Zymogen Granule Protein 16 Homolog B (ZG16B) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
GUESTBOOK