CLIA Kit for X-Ray Repair Cross Complementing 6 (XRCC6)

Ku70; CTC75; CTCBF; G22P1; ML8; TLAA; Lupus Ku Autoantigen Protein P70; ATP-Dependent DNA Helicase 2 Subunit 1; CTC box-binding factor 75 kDa; Thyroid-lupus autoantigen

  • CLIA Kit for X-Ray Repair Cross Complementing 6 (XRCC6) Packages (Simulation)
  • CLIA Kit for X-Ray Repair Cross Complementing 6 (XRCC6) Packages (Simulation)
  • CLIA Kit for X-Ray Repair Cross Complementing 6 (XRCC6) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for X-Ray Repair Cross Complementing 6 (XRCC6)

This assay has high sensitivity and excellent specificity for detection of X-Ray Repair Cross Complementing 6 (XRCC6).
No significant cross-reactivity or interference between X-Ray Repair Cross Complementing 6 (XRCC6) and analogues was observed.

Precision of the CLIA Kit for X-Ray Repair Cross Complementing 6 (XRCC6)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level X-Ray Repair Cross Complementing 6 (XRCC6) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level X-Ray Repair Cross Complementing 6 (XRCC6) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability of the CLIA Kit for X-Ray Repair Cross Complementing 6 (XRCC6)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for X-Ray Repair Cross Complementing 6 (XRCC6)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for X-Ray Repair Cross Complementing 6 (XRCC6)

The microplate provided in this kit has been pre-coated with an antibody specific to X-Ray Repair Cross Complementing 6 (XRCC6). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to X-Ray Repair Cross Complementing 6 (XRCC6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the X-Ray Repair Cross Complementing 6 (XRCC6) level in the sample or standard.;

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