CLIA Kit for Thyroid Hormone Responsive (THRSP)

S14; SPOT14; LPGP1; Lipogenic Protein 1; Thyroid hormone-inducible hepatic protein; Spot 14 protein

  • CLIA Kit for Thyroid Hormone Responsive (THRSP) Packages (Simulation)
  • CLIA Kit for Thyroid Hormone Responsive (THRSP) Packages (Simulation)
  • CLIA Kit for Thyroid Hormone Responsive (THRSP) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Thyroid Hormone Responsive (THRSP)

This assay has high sensitivity and excellent specificity for detection of Thyroid Hormone Responsive (THRSP).
No significant cross-reactivity or interference between Thyroid Hormone Responsive (THRSP) and analogues was observed.

Recovery of the CLIA Kit for Thyroid Hormone Responsive (THRSP)

Matrices listed below were spiked with certain level of recombinant Thyroid Hormone Responsive (THRSP) and the recovery rates were calculated by comparing the measured value to the expected amount of Thyroid Hormone Responsive (THRSP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-94 91
EDTA plasma(n=5) 78-102 97
heparin plasma(n=5) 79-90 84

Precision of the CLIA Kit for Thyroid Hormone Responsive (THRSP)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroid Hormone Responsive (THRSP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroid Hormone Responsive (THRSP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Thyroid Hormone Responsive (THRSP)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Thyroid Hormone Responsive (THRSP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-104% 81-99% 78-101% 97-105%
EDTA plasma(n=5) 84-92% 88-101% 81-101% 80-101%
heparin plasma(n=5) 90-101% 92-103% 81-102% 90-98%

Stability of the CLIA Kit for Thyroid Hormone Responsive (THRSP)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Thyroid Hormone Responsive (THRSP)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Thyroid Hormone Responsive (THRSP)

The microplate provided in this kit has been pre-coated with an antibody specific to Thyroid Hormone Responsive (THRSP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Thyroid Hormone Responsive (THRSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Thyroid Hormone Responsive (THRSP) level in the sample or standard.;

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPC790Hu01 Recombinant Thyroid Hormone Responsive (THRSP) Positive Control; Immunogen; SDS-PAGE; WB.
PAC790Hu01 Polyclonal Antibody to Thyroid Hormone Responsive (THRSP) WB; IHC
MAC790Hu22 Monoclonal Antibody to Thyroid Hormone Responsive (THRSP) WB; IHC; ICC; IP.
SEC790Hu ELISA Kit for Thyroid Hormone Responsive (THRSP) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC790Hu CLIA Kit for Thyroid Hormone Responsive (THRSP) Chemiluminescent immunoassay for Antigen Detection.
LMC790Hu Multiplex Assay Kit for Thyroid Hormone Responsive (THRSP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
GUESTBOOK