CLIA Kit for Ribonuclease A (RNase A)

RNASE1; RNASE; Rnase-A; RNase UpI-1; RIB-1; Ribonuclease,RNase A Family,1(Pancreatic)

  • CLIA Kit for Ribonuclease A (RNase A) Packages (Simulation)
  • CLIA Kit for Ribonuclease A (RNase A) Packages (Simulation)
  • CLIA Kit for Ribonuclease A (RNase A) Results demonstration
  • SCA297Bo.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Ribonuclease A (RNase A)

This assay has high sensitivity and excellent specificity for detection of Ribonuclease A (RNase A).
No significant cross-reactivity or interference between Ribonuclease A (RNase A) and analogues was observed.

Recovery of the CLIA Kit for Ribonuclease A (RNase A)

Matrices listed below were spiked with certain level of recombinant Ribonuclease A (RNase A) and the recovery rates were calculated by comparing the measured value to the expected amount of Ribonuclease A (RNase A) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-101 90
EDTA plasma(n=5) 89-103 93
heparin plasma(n=5) 88-102 98

Precision of the CLIA Kit for Ribonuclease A (RNase A)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribonuclease A (RNase A) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribonuclease A (RNase A) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Ribonuclease A (RNase A)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ribonuclease A (RNase A) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-103% 79-94% 85-103% 79-97%
EDTA plasma(n=5) 83-102% 89-97% 86-101% 93-101%
heparin plasma(n=5) 97-104% 79-102% 95-105% 95-103%

Stability of the CLIA Kit for Ribonuclease A (RNase A)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Ribonuclease A (RNase A)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Ribonuclease A (RNase A)

The microplate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A (RNase A). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ribonuclease A (RNase A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ribonuclease A (RNase A) level in the sample or standard.;

Related products

Catalog No. Organism species: Bos taurus; Bovine (Cattle) Applications (RESEARCH USE ONLY!)
URPA297Bo02 Recombinant Ribonuclease A (RNase A) Positive Control; Immunogen; SDS-PAGE; WB.
URPA297Bo01 Recombinant Ribonuclease A (RNase A) Positive Control; Immunogen; SDS-PAGE; WB.
UPAA297Bo02 Polyclonal Antibody to Ribonuclease A (RNase A) WB; IHC; ICC; IP.
UPAA297Bo01 Polyclonal Antibody to Ribonuclease A (RNase A) WB
ULAA297Bo71 Biotin-Linked Polyclonal Antibody to Ribonuclease A (RNase A) WB; IHC; ICC.
UMAA297Bo21 Monoclonal Antibody to Ribonuclease A (RNase A) WB; IHC; ICC; IP.
USEA297Bo ELISA Kit for Ribonuclease A (RNase A) Enzyme-linked immunosorbent assay for Antigen Detection.
USCA297Bo CLIA Kit for Ribonuclease A (RNase A) Chemiluminescent immunoassay for Antigen Detection.
ULMA297Bo Multiplex Assay Kit for Ribonuclease A (RNase A) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
GUESTBOOK