CLIA Kit for Platelet Factor 4 (PF4)
CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact
- Product No.SCA172Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range0.41-300pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.17pg/mL.
- Sample TypeSerum, platelet-poor plasma and cell culture supernates
- Download Instruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
-
FOB
US$ 588
For more details, please contact local distributors!US$ 840
For more details, please contact local distributors! US$ 3780
For more details, please contact local distributors! US$ 7140
For more details, please contact local distributors! US$ 58800
For more details, please contact local distributors!
Specificity of the CLIA Kit for Platelet Factor 4 (PF4)
This assay has high sensitivity and excellent specificity for detection of Platelet Factor 4 (PF4).
No significant cross-reactivity or interference between Platelet Factor 4 (PF4) and analogues was observed.
Recovery of the CLIA Kit for Platelet Factor 4 (PF4)
Matrices listed below were spiked with certain level of recombinant Platelet Factor 4 (PF4) and the recovery rates were calculated by comparing the measured value to the expected amount of Platelet Factor 4 (PF4) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 84-103 | 98 |
EDTA plasma(n=5) | 91-104 | 101 |
heparin plasma(n=5) | 93-102 | 96 |
Precision of the CLIA Kit for Platelet Factor 4 (PF4)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Factor 4 (PF4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Factor 4 (PF4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity of the CLIA Kit for Platelet Factor 4 (PF4)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Platelet Factor 4 (PF4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 86-95% | 83-97% | 84-92% | 95-102% |
EDTA plasma(n=5) | 89-96% | 89-96% | 95-104% | 86-104% |
heparin plasma(n=5) | 85-98% | 89-96% | 90-98% | 94-103% |
Stability of the CLIA Kit for Platelet Factor 4 (PF4)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the CLIA Kit for Platelet Factor 4 (PF4)
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
Test principle of the CLIA Kit for Platelet Factor 4 (PF4)
The microplate provided in this kit has been pre-coated with an antibody specific to Platelet Factor 4 (PF4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Platelet Factor 4 (PF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Platelet Factor 4 (PF4) level in the sample or standard.;
GIVEAWAYS
INCREMENT SERVICES
Single-component Reagents of Assay Kit
Lysis Buffer Specific for ELISA / CLIA
Quality Control of Kit
CLIA Kit Customized Service
Disease Model Customized Service
Serums Customized Service
TGFB1 Activation Reagent
Real Time PCR Experimental Service
Streptavidin
Fast blue Protein Stain solution
Single-component Reagents of FLIA Kit
Streptavidin-Agarose Beads
Related products
Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
APA172Hu01 | Active Platelet Factor 4 (PF4) | Cell culture; Activity Assays. |
RPA172Hu01 | Recombinant Platelet Factor 4 (PF4) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA172Hu02 | Recombinant Platelet Factor 4 (PF4) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA172Hu01 | Polyclonal Antibody to Platelet Factor 4 (PF4) | WB; IHC; ICC; IP. |
LAA172Hu71 | Biotin-Linked Polyclonal Antibody to Platelet Factor 4 (PF4) | WB; IHC; ICC. |
LAA172Hu81 | FITC-Linked Polyclonal Antibody to Platelet Factor 4 (PF4) | WB; IHC; ICC; IF. |
MAA172Hu21 | Monoclonal Antibody to Platelet Factor 4 (PF4) | WB; IHC; ICC; IP. |
MAA172Hu23 | Monoclonal Antibody to Platelet Factor 4 (PF4) | WB; IHC; ICC; IP. |
MAA172Hu24 | Monoclonal Antibody to Platelet Factor 4 (PF4) | WB; IHC; ICC; IP. |
MAA172Hu22 | Monoclonal Antibody to Platelet Factor 4 (PF4) | WB; IHC |
MAA172Hu25 | Monoclonal Antibody to Platelet Factor 4 (PF4) | WB; IHC; ICC; IP. |
SEA172Hu | ELISA Kit for Platelet Factor 4 (PF4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
AEA172Hu | ELISA Kit for Anti-Platelet Factor 4 Antibody (Anti-PF4) | Enzyme-linked immunosorbent assay for Antibody Detection. |
SCA172Hu | CLIA Kit for Platelet Factor 4 (PF4) | Chemiluminescent immunoassay for Antigen Detection. |
LMA172Hu | Multiplex Assay Kit for Platelet Factor 4 (PF4) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
PSA172Hu01 | Antibody Pair for Platelet Factor 4 (PF4) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA172Hu01 | ELISA Kit DIY Materials for Platelet Factor 4 (PF4) | Main materials for "Do It (ELISA Kit) Yourself". |