CLIA Kit for Iduronate-2-Sulfatase (IDS)

MPS2; SIDS; Hunter Syndrome; Idursulfase; Alpha-L-iduronate sulfate sulfatase

  • CLIA Kit for Iduronate-2-Sulfatase (IDS) Packages (Simulation)
  • CLIA Kit for Iduronate-2-Sulfatase (IDS) Packages (Simulation)
  • CLIA Kit for Iduronate-2-Sulfatase (IDS) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Iduronate-2-Sulfatase (IDS)

This assay has high sensitivity and excellent specificity for detection of Iduronate-2-Sulfatase (IDS).
No significant cross-reactivity or interference between Iduronate-2-Sulfatase (IDS) and analogues was observed.

Precision of the CLIA Kit for Iduronate-2-Sulfatase (IDS)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Iduronate-2-Sulfatase (IDS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Iduronate-2-Sulfatase (IDS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability of the CLIA Kit for Iduronate-2-Sulfatase (IDS)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Iduronate-2-Sulfatase (IDS)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Iduronate-2-Sulfatase (IDS)

The microplate provided in this kit has been pre-coated with an antibody specific to Iduronate-2-Sulfatase (IDS). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Iduronate-2-Sulfatase (IDS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Iduronate-2-Sulfatase (IDS) level in the sample or standard.;

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPH833Hu01 Recombinant Iduronate-2-Sulfatase (IDS) Positive Control; Immunogen; SDS-PAGE; WB.
PAH833Hu01 Polyclonal Antibody to Iduronate-2-Sulfatase (IDS) WB,IHC
MAH833Hu21 Monoclonal Antibody to Iduronate-2-Sulfatase (IDS) WB; IHC; ICC; IP.
SEH833Hu ELISA Kit for Iduronate-2-Sulfatase (IDS) Enzyme-linked immunosorbent assay for Antigen Detection.
SCH833Hu CLIA Kit for Iduronate-2-Sulfatase (IDS) Chemiluminescent immunoassay for Antigen Detection.
LMH833Hu Multiplex Assay Kit for Iduronate-2-Sulfatase (IDS) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
GUESTBOOK