This assay has high sensitivity and excellent specificity for detection of Hepcidin (Hepc).
No significant cross-reactivity or interference between Hepcidin (Hepc) and analogues was observed.

Matrices listed below were spiked with certain level of recombinant Hepcidin (Hepc) and the recovery rates were calculated by comparing the measured value to the expected amount of Hepcidin (Hepc) in samples.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepcidin (Hepc) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepcidin (Hepc) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hepcidin (Hepc) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Hepcidin (Hepc). A competitive inhibition reaction is launched between biotin labeled Hepcidin (Hepc) and unlabeled Hepcidin (Hepc) (Standards or samples) with the pre-coated antibody specific to Hepcidin (Hepc). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hepcidin (Hepc) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Hepcidin (Hepc) level in the sample or standard.

ELISA / CLIA Experiment Service

Single-component Reagents of Assay Kit
Lysis Buffer Specific for ELISA / CLIA
Quality Control of Kit
CLIA Kit Customized Service
Disease Model Customized Service
Serums Customized Service
TGFB1 Activation Reagent
Real Time PCR Experimental Service
Single-component Reagents of FLIA Kit