CLIA Kit for Heat Shock 70kDa Protein 5 (HSPA5)
MIF2; BIP; GRP78; Immunoglobulin heavy chain-binding protein; Glucose Regulated Protein 78; Binding Immunoglobulin Protein; Endoplasmic reticulum lumenal Ca binding grp78
- Product No.SCC343Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.055ng/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Download Instruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the CLIA Kit for Heat Shock 70kDa Protein 5 (HSPA5)
This assay has high sensitivity and excellent specificity for detection of Heat Shock 70kDa Protein 5 (HSPA5).
No significant cross-reactivity or interference between Heat Shock 70kDa Protein 5 (HSPA5) and analogues was observed.
Recovery of the CLIA Kit for Heat Shock 70kDa Protein 5 (HSPA5)
Matrices listed below were spiked with certain level of recombinant Heat Shock 70kDa Protein 5 (HSPA5) and the recovery rates were calculated by comparing the measured value to the expected amount of Heat Shock 70kDa Protein 5 (HSPA5) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-92 | 87 |
| EDTA plasma(n=5) | 95-104 | 101 |
| heparin plasma(n=5) | 86-95 | 90 |
Precision of the CLIA Kit for Heat Shock 70kDa Protein 5 (HSPA5)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity of the CLIA Kit for Heat Shock 70kDa Protein 5 (HSPA5)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heat Shock 70kDa Protein 5 (HSPA5) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-104% | 82-104% | 97-105% | 91-103% |
| EDTA plasma(n=5) | 88-104% | 86-94% | 98-105% | 86-94% |
| heparin plasma(n=5) | 90-99% | 79-99% | 78-97% | 91-99% |
Stability of the CLIA Kit for Heat Shock 70kDa Protein 5 (HSPA5)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the CLIA Kit for Heat Shock 70kDa Protein 5 (HSPA5)
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
Test principle of the CLIA Kit for Heat Shock 70kDa Protein 5 (HSPA5)
The microplate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 5 (HSPA5). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 5 (HSPA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Heat Shock 70kDa Protein 5 (HSPA5) level in the sample or standard.;
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