CLIA Kit for Erythroferrone (ERFE)
FAM132B; C1QTNF15; CTRP15; Myonectin; Family With Sequence Similarity 132, Member B; Complement C1q tumor necrosis factor-related protein 15
- Product No.SCU540Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range13.7-10,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.3pg/mL.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Download Instruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the CLIA Kit for Erythroferrone (ERFE)
This assay has high sensitivity and excellent specificity for detection of Erythroferrone (ERFE).
No significant cross-reactivity or interference between Erythroferrone (ERFE) and analogues was observed.
Recovery of the CLIA Kit for Erythroferrone (ERFE)
Matrices listed below were spiked with certain level of recombinant Erythroferrone (ERFE) and the recovery rates were calculated by comparing the measured value to the expected amount of Erythroferrone (ERFE) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 84-101 | 89 |
| EDTA plasma(n=5) | 95-104 | 101 |
| heparin plasma(n=5) | 89-101 | 96 |
Precision of the CLIA Kit for Erythroferrone (ERFE)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Erythroferrone (ERFE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Erythroferrone (ERFE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity of the CLIA Kit for Erythroferrone (ERFE)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Erythroferrone (ERFE) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-94% | 86-102% | 84-101% | 83-103% |
| EDTA plasma(n=5) | 99-105% | 88-102% | 91-99% | 81-97% |
| heparin plasma(n=5) | 94-102% | 93-101% | 98-105% | 79-105% |
Stability of the CLIA Kit for Erythroferrone (ERFE)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the CLIA Kit for Erythroferrone (ERFE)
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
Test principle of the CLIA Kit for Erythroferrone (ERFE)
The microplate provided in this kit has been pre-coated with an antibody specific to Erythroferrone (ERFE). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Erythroferrone (ERFE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Erythroferrone (ERFE) level in the sample or standard.;
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Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPU540Hu01 | Recombinant Erythroferrone (ERFE) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPU540Hu02 | Recombinant Erythroferrone (ERFE) | Positive Control; Immunogen; SDS-PAGE; WB. |
| SEU540Hu | ELISA Kit for Erythroferrone (ERFE) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCU540Hu | CLIA Kit for Erythroferrone (ERFE) | Chemiluminescent immunoassay for Antigen Detection. |
| LMU540Hu | Multiplex Assay Kit for Erythroferrone (ERFE) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |