CLIA Kit for Arginine (Arg)

(S)-2-Amino-5-Guanidinopentanoic Acid

  • CLIA Kit for Arginine (Arg) Packages (Simulation)
  • CLIA Kit for Arginine (Arg) Packages (Simulation)
  • CLIA Kit for Arginine (Arg) Results demonstration
  • CCB938Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Arginine (Arg)

This assay has high sensitivity and excellent specificity for detection of Arginine (Arg).
No significant cross-reactivity or interference between Arginine (Arg) and analogues was observed.

Recovery of the CLIA Kit for Arginine (Arg)

Matrices listed below were spiked with certain level of Arginine (Arg) and the recovery rates were calculated by comparing the measured value to the expected amount of Arginine (Arg) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-104 96
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 88-102 95

Precision of the CLIA Kit for Arginine (Arg)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arginine (Arg) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arginine (Arg) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Arginine (Arg)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Arginine (Arg) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-98% 99-105% 78-99% 90-102%
EDTA plasma(n=5) 94-101% 79-103% 79-90% 79-94%
heparin plasma(n=5) 83-91% 80-88% 81-95% 98-105%

Stability of the CLIA Kit for Arginine (Arg)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Arginine (Arg)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.

Test principle of the CLIA Kit for Arginine (Arg)

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Arginine (Arg). A competitive inhibition reaction is launched between biotin labeled Arginine (Arg) and unlabeled Arginine (Arg) (Standards or samples) with the pre-coated antibody specific to Arginine (Arg). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Arginine (Arg) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Arginine (Arg) level in the sample or standard.

Related products

Catalog No. Organism species: Pan-species (General) Applications (RESEARCH USE ONLY!)
UCPB938Ge11 BSA Conjugated Arginine (Arg) Immunogen; SDS-PAGE; WB.
UCPB938Ge21 OVA Conjugated Arginine (Arg) Immunogen; SDS-PAGE; WB.
UPAB938Ge01 Polyclonal Antibody to Arginine (Arg) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
UCEB938Ge ELISA Kit for Arginine (Arg) Enzyme-linked immunosorbent assay for Antigen Detection.
UCCB938Ge CLIA Kit for Arginine (Arg) Chemiluminescent immunoassay for Antigen Detection.
ULMB938Ge Multiplex Assay Kit for Arginine (Arg) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
UKSB938Ge11 ELISA Kit DIY Materials for Arginine (Arg) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK