Biotin-Linked Polyclonal Antibody to Leptin (LEP)
OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein
- Product No.ULAA084Bo71
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- SourceAntibody labeling
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody PAA084Bo01-Polyclonal Antibody to Leptin (LEP)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC.
 If the antibody is used in flow cytometry, please check FCM antibodies.
- Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- 
    FOB
    
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SPECIFITY of the Biotin-Linked Polyclonal Antibody to Leptin (LEP)
The antibody is a rabbit polyclonal antibody raised against LEP. It has been selected for its ability to recognize LEP in immunohistochemical staining and western blotting.
USAGE of the Biotin-Linked Polyclonal Antibody to Leptin (LEP)
            Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.        
STORAGE of the of the Biotin-Linked Polyclonal Antibody to Leptin (LEP)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Biotin-Linked Polyclonal Antibody to Leptin (LEP)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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INCREMENT SERVICES
            Protein A/G Purification Column
                    Staining Solution for Cells and Tissue
                    Positive Control  for Antibody
                    Tissue/Sections Customized Service
                    Phosphorylated Antibody Customized Service
                    Western Blot (WB) Experiment Service
                    Immunohistochemistry (IHC) Experiment Service
                    Immunocytochemistry (ICC) Experiment Service
                    Flow Cytometry (FCM) Experiment Service
                    Immunoprecipitation (IP) Experiment Service
                    Immunofluorescence (IF) Experiment Service
                    Buffer
                    DAB Chromogen Kit
                    SABC Kit
                    Real Time PCR Experimental Service
                            
Related products
| Catalog No. | Organism species: Bos taurus; Bovine (Cattle) | Applications (RESEARCH USE ONLY!) | 
| URPA084Bo01 | Recombinant Leptin (LEP) | Positive Control; Immunogen; SDS-PAGE; WB. | 
| UPAA084Bo01 | Polyclonal Antibody to Leptin (LEP) | IHC | 
| ULAA084Bo71 | Biotin-Linked Polyclonal Antibody to Leptin (LEP) | WB; IHC; ICC. | 
| UMAA084Bo21 | Monoclonal Antibody to Leptin (LEP) | WB; IHC; ICC; IP. | 
| USEA084Bo | ELISA Kit for Leptin (LEP) | Enzyme-linked immunosorbent assay for Antigen Detection. | 
| ULMA084Bo | Multiplex Assay Kit for Leptin (LEP) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. | 
| UPSA084Bo01 | Antibody Pair for Leptin (LEP) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. | 
| UKSA084Bo01 | ELISA Kit DIY Materials for Leptin (LEP) | Main materials for "Do It (ELISA Kit) Yourself". | 

 
                     
                    