Polyclonal Antibody to Vimentin (VIM)
- Product No.UPAB040Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPB040Hu01-Recombinant Vimentin (VIM)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/mL
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus)
- ApplicationsWB; IHC; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 50
For more details, please contact local distributors!US$ 116
For more details, please contact local distributors! US$ 166
For more details, please contact local distributors! US$ 415
For more details, please contact local distributors! US$ 1660
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Prostate Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human VIM Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Placenta Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Cerebrum Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Uterus Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Small intestine Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Lymph node Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Lung Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Pancreas Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Spleen Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Colon Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Spinal cord Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Small intestine Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human VIM Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Lung Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human VIM Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Spleen Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human VIM Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Sample: MCF7 cell
Primary Ab: 10µg/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
AF488 staining on IF;
Sample: HepG2 cell
Primary Ab: 20µg/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Western Blot; Samples: Lane1: Hela cell lysate; Lane2: A549 cell lysate; Primary Ab: 0.1µg/ml Rabbit Anti-Human VIM Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Rat Heart lysate; Lane3: Rat Cerebrum lysate; Lane4: 293T cell lysate; Lane5: Hela cell lysate
Primary Ab: 0.1µg/ml Rabbit Anti-Human VIM Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: 293T cell lysate; Lane3: Hela cell lysate
Primary Ab: 1μg/ml Rabbit Anti-Human VIM Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Canine Heart lysate; Lane2: Porcine Heart lysate; Lane3: Bovine Heart lysate; Lane4: Caprine Heart lysate; Lane5: Equine Heart lysate; Lane6: Gallus Heart lysate; Lane7: Cavia Heart lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Human VIM Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Rat Heart lysate; Lane3: 293T cell lysate; Lane4: Hela cell lysate
Primary Ab: 0.1µg/ml Rabbit Anti-Human VIM Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Vimentin (VIM)
The antibody is a rabbit polyclonal antibody raised against VIM. It has been selected for its ability to recognize VIM in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Vimentin (VIM)
Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Vimentin (VIM)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Vimentin (VIM)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
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| UMAB040Hu23 | Monoclonal Antibody to Vimentin (VIM) | WB; IHC; ICC; IP. |
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| UFAB040Hu02 | Anti-Vimentin (VIM) Monoclonal Antibody | Flow cytometry. |
| UFAB040Hu06 | Anti-Vimentin (VIM) Monoclonal Antibody | Flow cytometry. |
| UFAB040Hu04 | Anti-Vimentin (VIM) Monoclonal Antibody | Flow cytometry. |
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| USEB040Hu | ELISA Kit for Vimentin (VIM) | Enzyme-linked immunosorbent assay for Antigen Detection. |
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