Monoclonal Antibody to Thymidine Kinase 1, Soluble (TK1)
TK2; Thymidine kinase, cytosolic
- Product No.MAC823Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)C3
- Ig Isotype IgG2b Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPC823Hu01-Recombinant Thymidine Kinase 1, Soluble (TK1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species MoreSus scrofa; Porcine (Pig)
- ApplicationsWB,ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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For more details, please contact local distributors!US$ 259
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For more details, please contact local distributors! US$ 3700
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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FITC staining on IF; Samples: Human MCF7 cell; Primary Ab: 20µg/ml Mouse Anti-Human TK1 Antibody Second Ab: 1µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
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Figure. Western Blot; Sample: Recombinant TK1, Human.
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Knockout Varification:
Lane 1: Wild-type MCF7 cell lysate;
Lane 2: TK1 knockout MCF7 cell lysate;
Predicted MW: 25kDa
Observed MW: 25kDa
Primary Ab: 6µg/ml Mouse Anti-Human TK1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Hela cell lysate
Primary Ab: 6µg/ml Mouse Anti-Human TK1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) Selected -
Western Blot; Sample: MCF7 cell lysate
Primary Ab: 6µg/ml Mouse Anti-Human TK1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) Selected -
Western Blot; Sample: 293T cell lysate
Primary Ab: 6µg/ml Mouse Anti-Human TK1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) Selected -
Western Blot; Sample: Porcine Lymph node lysate
Primary Ab: 6µg/ml Mouse Anti-Human TK1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) Selected
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Monoclonal Antibody to Thymidine Kinase 1, Soluble (TK1)
The antibody is a mouse monoclonal antibody raised against TK1. It has been selected for its ability to recognize TK1 in immunohistochemical staining and western blotting.
USAGE of the Monoclonal Antibody to Thymidine Kinase 1, Soluble (TK1)
Western blotting: 0.01-6µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Monoclonal Antibody to Thymidine Kinase 1, Soluble (TK1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Monoclonal Antibody to Thymidine Kinase 1, Soluble (TK1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service