Polyclonal Antibody to Pyruvate Dehydrogenase Beta (PDHb)
PDH-B; PHE1B; PHE1-B; Pyruvate Dehydrogenase(lipoamide)Beta
- Product No.PAB923Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPB923Hu01-Recombinant Pyruvate Dehydrogenase Beta (PDHb)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
- ApplicationsWB; IHC; ICC; IP; FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 100
For more details, please contact local distributors!US$ 232
For more details, please contact local distributors! US$ 332
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For more details, please contact local distributors! US$ 3320
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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IHC Images
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FITC staining on IF;
Sample: Human Hela cell;
Primary Ab: 20ug/ml Rabbit Anti-Human PDHb Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
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Figure. Western Blot; Sample: Recombinant PDHb, Human.
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Western Blot; Sample: Human Serum;
Primary Ab: 1µg/ml Rabbit Anti-Human PDHb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Samples: Lane1: Human Lung lysate; Lane2: Porcine Liver lysate; Lane3: Rat Cerebrum lysate; Lane4: Rat Heart lysate; Lane5: HepG2 cell lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Human PDHb Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Liver lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human PDHb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Cerebrum lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human PDHb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Lung lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human PDHb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human Hela cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human PDHb Polyclonal Antibody (Catalog PAB923Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Pyruvate Dehydrogenase Beta (PDHb)
The antibody is a rabbit polyclonal antibody raised against PDHb. It has been selected for its ability to recognize PDHb in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Pyruvate Dehydrogenase Beta (PDHb)
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Pyruvate Dehydrogenase Beta (PDHb)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Pyruvate Dehydrogenase Beta (PDHb)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPB923Hu01 | Recombinant Pyruvate Dehydrogenase Beta (PDHb) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB923Hu01 | Polyclonal Antibody to Pyruvate Dehydrogenase Beta (PDHb) | WB; IHC; ICC; IP; FCM |
| MAB923Hu22 | Monoclonal Antibody to Pyruvate Dehydrogenase Beta (PDHb) | WB; IHC; ICC; IP. |
| SEB923Hu | ELISA Kit for Pyruvate Dehydrogenase Beta (PDHb) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB923Hu | Multiplex Assay Kit for Pyruvate Dehydrogenase Beta (PDHb) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSB923Hu01 | ELISA Kit DIY Materials for Pyruvate Dehydrogenase Beta (PDHb) | Main materials for "Do It (ELISA Kit) Yourself". |