Polyclonal Antibody to Otubain 1 (OTUB1)

HSPC263; OTB1; OTU1; OTU Domain,Ubiquitin Aldehyde Binding 1; Deubiquitinating enzyme OTUB1; Ubiquitin-specific-processing protease OTUB1

  • Polyclonal Antibody to Otubain 1 (OTUB1) Packages (Simulation)
  • Polyclonal Antibody to Otubain 1 (OTUB1) Packages (Simulation)
  • Polyclonal Antibody to Otubain 1 (OTUB1) DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human OTUB1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Otubain 1 (OTUB1) DAB staining on IHC-P;
    Sample: Mouse Liver Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human OTUB1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Otubain 1 (OTUB1) DAB staining on IHC-P;
    Sample: Mouse Kidney Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human OTUB1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Otubain 1 (OTUB1) AF488 staining on IF;
    Sample: Hela cell
    Primary Ab: 20µg/ml Rabbit Anti-Human OTUB1 Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAL838Hu01.jpg Figure. Western Blot; Sample: Recombinant OTUB1, Human.
  • Polyclonal Antibody to Otubain 1 (OTUB1) Western Blot; Samples: Lane1: Mouse Testis lysate; Lane2: Rat Testis lysate; Lane3: Porcine Cerebrum lysate; Lane4: Hela cell lysate;
    Primary Ab: 0.1µg/ml Rabbit Anti-Human OTUB1 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Otubain 1 (OTUB1) Human MCF7 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human OTUB1 Polyclonal Antibody (Catalog PAL838Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Otubain 1 (OTUB1)

The antibody is a rabbit polyclonal antibody raised against OTUB1. It has been selected for its ability to recognize OTUB1 in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Otubain 1 (OTUB1)

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/mL;
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Otubain 1 (OTUB1)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Otubain 1 (OTUB1)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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URPL838Hu01 Recombinant Otubain 1 (OTUB1) Positive Control; Immunogen; SDS-PAGE; WB.
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