Polyclonal Antibody to Nucleolin (NCL)
C23; Protein C23
- Product No.UPAC242Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPC242Hu01-Recombinant Nucleolin (NCL)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 53
For more details, please contact local distributors!US$ 123
For more details, please contact local distributors! US$ 175
For more details, please contact local distributors! US$ 438
For more details, please contact local distributors! US$ 1750
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Mastadenoma Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human NCL Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Vector Red staining on IHC-P;
Samples: Human Tonsil Tissue;
Primary Ab: 15µg/ml Rabbit Anti-Human NCL Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody -
Vector Red staining on IHC-P;
Samples: Human Uterus Tissue;
Primary Ab: 15µg/ml Rabbit Anti-Human NCL Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody -
AF488 staining on IF;
Sample: A431 cell
Primary Ab: 20µg/ml Rabbit Anti-Human NCL Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant NCL, Human.
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Western Blot; Samples: Lane1: K562 cell lysate; Lane2: 293T cell lysate; Lane3: Jurkat cell lysate;
Primary Ab: 0.5µg/ml Rabbit Anti-Human NCL Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Nucleolin (NCL)
The antibody is a rabbit polyclonal antibody raised against NCL. It has been selected for its ability to recognize NCL in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Nucleolin (NCL)
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Nucleolin (NCL)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Nucleolin (NCL)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| URPC242Hu01 | Recombinant Nucleolin (NCL) | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC242Hu02 | Recombinant Nucleolin (NCL) | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC242Hu03 | Recombinant Nucleolin (NCL) | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAC242Hu02 | Polyclonal Antibody to Nucleolin (NCL) | WB; IHC; ICC; IP. |
| UPAC242Hu03 | Polyclonal Antibody to Nucleolin (NCL) | WB; IHC; ICC; IP. |
| UPAC242Hu01 | Polyclonal Antibody to Nucleolin (NCL) | WB; IHC; ICC; IP. |
| UMAC242Hu22 | Monoclonal Antibody to Nucleolin (NCL) | WB; IHC; ICC; IP. |
| USEC242Hu | ELISA Kit for Nucleolin (NCL) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMC242Hu | Multiplex Assay Kit for Nucleolin (NCL) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |