Polyclonal Antibody to Cytokeratin 8 (CK8)

K8; CARD2; KRT8; CYK8; K2C8; KO; Keratin 8; Keratin, type II cytoskeletal 8

  • Polyclonal Antibody to Cytokeratin 8 (CK8) Packages (Simulation)
  • Polyclonal Antibody to Cytokeratin 8 (CK8) Packages (Simulation)
  • Polyclonal Antibody to Cytokeratin 8 (CK8) DAB staining on IHC-P; Samples: Human Liver cancer Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human CK8 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cytokeratin 8 (CK8) DAB staining on IHC-P;
    Samples: Human Tonsil Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cytokeratin 8 (CK8) DAB staining on IHC-P;
    Samples: Human Prostate Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cytokeratin 8 (CK8) DAB staining on IHC-P;
    Samples: Human Thyroid cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cytokeratin 8 (CK8) DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cytokeratin 8 (CK8) DAB staining on IHC-P;
    Samples: Human Stomach Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human KRT8 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cytokeratin 8 (CK8) FITC staining on IF;
    Samples: Human MCF7 cell;
    Primary Ab: 20μg/ml Rabbit Anti-Human KRT8 Antibody
    Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • PAC025Hu01.jpg Figure. Western Blot; Sample: Recombinant CK8, Human.
  • Polyclonal Antibody to Cytokeratin 8 (CK8) Knockout Varification:
    Lane 1: Wild-type Hela cell lysate;
    Lane 2: KRT8 knockout Hela cell lysate;
    Predicted MW: 54,57kDa
    Observed MW: 57kDa
    Primary Ab: 2µg/ml Rabbit Anti-Human KRT8 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cytokeratin 8 (CK8) Western Blot; Sample: Lane1: A431 cell lysate; Lane2: A549 cell lysate; Lane3: Hela cell lysate; Lane4: MCF7 cell lysate
    Primary Ab: 0.05µg/ml Rabbit Anti-Human CK8 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cytokeratin 8 (CK8) Human MCF7 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human CK8 Polyclonal Antibody (Catalog PAC025Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Cytokeratin 8 (CK8)

The antibody is a rabbit polyclonal antibody raised against CK8. It has been selected for its ability to recognize CK8 in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Cytokeratin 8 (CK8)

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Cytokeratin 8 (CK8)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Cytokeratin 8 (CK8)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPC025Hu01 Recombinant Cytokeratin 8 (CK8) Positive Control; Immunogen; SDS-PAGE; WB.
PAC025Hu02 Polyclonal Antibody to Cytokeratin 8 (CK8) WB; IHC; ICC; IP.
PAC025Hu01 Polyclonal Antibody to Cytokeratin 8 (CK8) WB,IHC,ICC/IF; FCM
LAC025Hu81 FITC-Linked Polyclonal Antibody to Cytokeratin 8 (CK8) WB; IHC; ICC; IF.
LAC025Hu71 Biotin-Linked Polyclonal Antibody to Cytokeratin 8 (CK8) WB; IHC; ICC.
MAC025Hu21 Monoclonal Antibody to Cytokeratin 8 (CK8) WB; IHC; ICC; IP.
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MAC025Hu22 Monoclonal Antibody to Cytokeratin 8 (CK8) WB; IHC; ICC; IP.
SEC025Hu ELISA Kit for Cytokeratin 8 (CK8) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC025Hu Multiplex Assay Kit for Cytokeratin 8 (CK8) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
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