Polyclonal Antibody to Cytokeratin 7 (CK7)
KRT7; K2C7; K7; SCL; Keratin 7; Sarcolectin; Keratin,55K Type II Cytoskeletal; Keratin, type II cytoskeletal 7
- Product No.PAA556Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA556Hu01-Recombinant Cytokeratin 7 (CK7)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/ml
- Organism Species MoreRattus norvegicus (Rat)
- ApplicationsWB,IHC,ICC/IF,FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 95
For more details, please contact local distributors!US$ 221
For more details, please contact local distributors! US$ 316
For more details, please contact local distributors! US$ 790
For more details, please contact local distributors! US$ 3160
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Lung cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human KRT7 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Rat Uterus Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human CK7 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Kidney Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human CK7 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Parotid Gland Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human CK7 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Lung Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human CK7 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Pancreas Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human CK7 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 10ug/ml Rabbit Anti-Human CK7 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
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Figure. Western Blot; Sample: Recombinant KRT7, Human.
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Western Blot; Samples: Lane1: Human Lung lysate; Lane2: Rat Liver lysate; Lane3: A549 cell lysate; Lane4: HepG2 cell lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Human CK7 Antibody
Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Saliva
Primary Ab: 0.1µg/ml Rabbit Anti-Human CK7 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human Hela cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human CK7 Polyclonal Antibody (Catalog PAA556Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Cytokeratin 7 (CK7)
The antibody is a rabbit polyclonal antibody raised against CK7. It has been selected for its ability to recognize CK7 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Cytokeratin 7 (CK7)
Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence:5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Cytokeratin 7 (CK7)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Cytokeratin 7 (CK7)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service