Polyclonal Antibody to Interferon Regulatory Factor 3 (IRF3)
- Product No.PAB589Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPB589Hu01-Recombinant Interferon Regulatory Factor 3 (IRF3)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/mL
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
- ApplicationsWB; IHC; ICC; IP; FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 100
For more details, please contact local distributors!US$ 232
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Packages (Simulation)
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Packages (Simulation)
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Used in DAB staining on fromalin fixed paraffin-embedded Kidney tissue
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Figure: FITC staining on IHC-P;
Sample: Hela cells. -
AF488 staining on IF;
Sample: Hepa1-6 cell
Primary Ab: 20µg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11) -
AF488 staining on IF;
Sample: NIH/3T3 cell
Primary Ab: 20µg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Western Blot; Sample: Lane1: 3T3-L1 cell lysate; Lane2: Rat Kidney lysate Primary Ab: 0.6µg/ml Rabbit Anti-Human IRF3 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 3µg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Hela cell lysate
Primary Ab: 0.3µg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human IRF3 Polyclonal Antibody (Catalog PAB589Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Interferon Regulatory Factor 3 (IRF3)
The antibody is a rabbit polyclonal antibody raised against IRF3. It has been selected for its ability to recognize IRF3 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Interferon Regulatory Factor 3 (IRF3)
Western blotting: 0.01-3μg/mL
Immunohistochemistry: 5-30μg/mL
Immunocytochemistry: 5-30μg/mL
Flow cytometry:20μg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Interferon Regulatory Factor 3 (IRF3)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Interferon Regulatory Factor 3 (IRF3)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPB589Hu01 | Recombinant Interferon Regulatory Factor 3 (IRF3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB589Hu01 | Polyclonal Antibody to Interferon Regulatory Factor 3 (IRF3) | WB; IHC; ICC; IP; FCM |
| MAB589Hu22 | Monoclonal Antibody to Interferon Regulatory Factor 3 (IRF3) | WB; IHC; ICC; IP. |
| SEB589Hu | ELISA Kit for Interferon Regulatory Factor 3 (IRF3) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB589Hu | Multiplex Assay Kit for Interferon Regulatory Factor 3 (IRF3) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |