Polyclonal Antibody to Tumor Protein p53 (P53)
TP53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
- Product No.UPAA928Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA928Hu01-Recombinant Tumor Protein p53 (P53)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/mL
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
- ApplicationsWB; IHC; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 47
For more details, please contact local distributors!US$ 111
For more details, please contact local distributors! US$ 158
For more details, please contact local distributors! US$ 395
For more details, please contact local distributors! US$ 1580
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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FITC staining on IF; Samples: Human HepG2 cell; Primary Ab: 20µg/ml Rabbit Anti-Human TP53 Antibody Second Ab: 1.5µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
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DAB staining on IHC-P;
Sample: Porcine Colon Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human P53 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Lymph node Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human P53 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure 1. Western Blot; Sample: Recombinant TP53, Human.
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Western Blot; Sample: Lane1: Rat Spleen lysate; Lane2: Human 293T cell lysate
Primary Ab: 1µg/mL Rabbit Anti-Human TP53 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type MCF7 cell lysate;
Lane 2: TP53 knockout MCF7 cell lysate;
Predicted MW: 24,30,33,34,38,39,44kDa
Observed MW: 50kDa
Primary Ab: 1µg/ml Rabbit Anti-Human TP53 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: A431 cell lysate; Lane2: MCF7 cell lysate
Primary Ab: 0.3µg/ml Rabbit Anti-Human TP53 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Porcine Skin lysate
Primary Ab: 0.3µg/ml Rabbit Anti-Human TP53 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Tumor Protein p53 (P53)
The antibody is a rabbit polyclonal antibody raised against P53. It has been selected for its ability to recognize P53 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Tumor Protein p53 (P53)
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Tumor Protein p53 (P53)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Tumor Protein p53 (P53)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service