Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1)
HMG1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein
- Product No.UMAA399Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)C4
- Ig Isotype IgG1 Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPA399Hu01-Recombinant High Mobility Group Protein 1 (HMGB1)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species MoreRattus norvegicus (Rat)
- ApplicationsWB;ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 50
For more details, please contact local distributors!US$ 118
For more details, please contact local distributors! US$ 168
For more details, please contact local distributors! US$ 420
For more details, please contact local distributors! US$ 1680
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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FITC staining on IF; Samples: Human HepG2 Cells; Primary Ab: 40µg/ml Mouse Anti-Human HMG1 Antibody Second Ab: 1µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
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AF488 staining on IF;
Sample: Hela cell
Primary Ab: 30µg/ml Mouse Anti-Human HMGB1 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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Figure. Western Blot; Sample: Recombinant HMG1, Human.
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Figure.Western Blot
Human Jurkat Cells
Primary Ab: 3µg/mL Rabbit Anti-Human HMG1 Ab
Second Ab: 1:5000 Dilution of HRP-Linked Rabbit
Anti-Mouse IgG Ab (Catalog: SAA544Mu09) -
Western Blot; Sample: Human Lung lysate;
Primary Ab: 2µg/ml Mouse Anti-Human HMG1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 2µg/ml Mouse Anti-Human HMG1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Rat Lung lysate; Lane2: Rat Cerebrum lysate; Lane3: Jurkat cell lysate; Lane4: Hela cell lysate
Primary Ab: 0.2µg/ml Mouse Anti-Human HMGB1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1)
The antibody is a mouse monoclonal antibody raised against HMGB1. It has been selected for its ability to recognize HMGB1 in immunohistochemical staining and western blotting.
USAGE of the Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1)
Western blotting: 0.01-3µg/mL;
Immunocytochemistry: 5-40µg/mL;
Optimal working dilutions must be determined by end user
STORAGE of the of the Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service