Polyclonal Antibody to Enolase, Neuron Specific (NSE)
ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase
- Product No.PAA537Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA537Ra01-Recombinant Enolase, Neuron Specific (NSE)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/mL
- Organism Species MoreHomo sapiens (Human), Mus musculus (Mouse)
- ApplicationsWB; IHC; FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 103
For more details, please contact local distributors!US$ 239
For more details, please contact local distributors! US$ 342
For more details, please contact local distributors! US$ 855
For more details, please contact local distributors! US$ 3420
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Rat Pancreas Tissue; Primary Ab: 20µg/ml Rabbit Anti-Rat NSE Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Rat Cerebrum Tissue;
Primary Ab: 20µg/mL Rabbit Anti-Rat NSE Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Rat Testis Tissue;
Primary Ab: 20µg/mL Rabbit Anti-Rat NSE Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant NSE, Rat.
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Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Mouse Cerebrum lysate; Lane3: HepG2 cell lysate; Lane4: Hela cell lysate
Primary Ab: 1µg/mL Rabbit Anti-Rat NSE Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type HepG2 cell lysate;
Lane 2: NSE knockout HepG2 cell lysate;
Predicted MW: 47kd
Observed MW: 52kd
Primary Ab: 1µg/mL Rabbit Anti-Rat NSE Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Mouse Hepa1-6 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/mL rabbit Anti-Mouse NSE Polyclonal Antibody (Catalog PAA537Ra01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/mL FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Enolase, Neuron Specific (NSE)
The antibody is a rabbit polyclonal antibody raised against NSE. It has been selected for its ability to recognize NSE in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Enolase, Neuron Specific (NSE)
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Enolase, Neuron Specific (NSE)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Enolase, Neuron Specific (NSE)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| RPA537Ra01 | Recombinant Enolase, Neuron Specific (NSE) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA537Ra01 | Polyclonal Antibody to Enolase, Neuron Specific (NSE) | WB; IHC; FCM |
| FAA537Ra01 | Anti-Enolase, Neuron Specific (NSE) Polyclonal Antibody | Flow cytometry. |
| LAA537Ra81 | FITC-Linked Polyclonal Antibody to Enolase, Neuron Specific (NSE) | WB; IHC; ICC; IF. |
| LAA537Ra71 | Biotin-Linked Polyclonal Antibody to Enolase, Neuron Specific (NSE) | WB; IHC; ICC. |
| MAA537Ra21 | Monoclonal Antibody to Enolase, Neuron Specific (NSE) | WB; IHC; ICC; IP. |
| SEA537Ra | ELISA Kit for Enolase, Neuron Specific (NSE) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA537Ra | Mini Samples ELISA Kit for Enolase, Neuron Specific (NSE) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA537Ra | Multiplex Assay Kit for Enolase, Neuron Specific (NSE) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |