Polyclonal Antibody to Neutrophil Elastase (NE)
ELANE; HLE; HNE; ELA2; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Leukocyte Elastase; PMN Elastase
- Product No.PAA181Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA181Hu01-Recombinant Neutrophil Elastase (NE)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 95
For more details, please contact local distributors!US$ 221
For more details, please contact local distributors! US$ 316
For more details, please contact local distributors! US$ 790
For more details, please contact local distributors! US$ 3160
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Lung cancer Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human ELA2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Rectum Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human ELA2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Vector Red staining on IHC-P;
Samples: Human Spleen Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human ELA2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody -
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human NE Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant ELA2, Human.
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Western Blot; Sample: Human Leukocyte lysate
Primary Ab: 2µg/ml Rabbit Anti-Human NE Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Neutrophil Elastase (NE)
The antibody is a rabbit polyclonal antibody raised against NE. It has been selected for its ability to recognize NE in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Neutrophil Elastase (NE)
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Neutrophil Elastase (NE)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Neutrophil Elastase (NE)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPA181Hu02 | Recombinant Neutrophil Elastase (NE) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA181Hu03 | Active Neutrophil Elastase (NE) | Cell culture; Activity Assays. |
| RPA181Hu01 | Recombinant Neutrophil Elastase (NE) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA181Hu03 | Recombinant Neutrophil Elastase (NE) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA181Hu04 | Recombinant Neutrophil Elastase (NE) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA181Hu01 | Active Neutrophil Elastase (NE) | Cell culture; Activity Assays. |
| PAA181Hu01 | Polyclonal Antibody to Neutrophil Elastase (NE) | WB; IHC; ICC; IP. |
| MAA181Hu22 | Monoclonal Antibody to Neutrophil Elastase (NE) | WB; IHC; ICC; IP. |
| SEA181Hu | ELISA Kit for Neutrophil Elastase (NE) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA181Hu | Mini Samples ELISA Kit for Neutrophil Elastase (NE) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA181Hu | Multiplex Assay Kit for Neutrophil Elastase (NE) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |