Active Transforming Growth Factor Beta 1 (TGFb1)
TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide
- Product No.APA124Bo01
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point7.2
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 302
For more details, please contact local distributors!US$ 756
For more details, please contact local distributors! US$ 1512
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For more details, please contact local distributors! US$ 11340
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Transforming Growth Factor Beta 1 (TGFb1)
Transforming growth factor beta 1 (TGF-β1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. It is reported that TGF-β1 can stimulate cell transformation by Smad signal transduction .To test the bioactivity of TGF-β1, A549 cells were seeded into 24-well plate at a density of 1x106 cells/mL, and allowed to attach overnight before treated with certain concentrations of TGF-β1. After 48 hours, the morphological changes of A549 were observed by inverted microscope. The results was shown in Figure 1. A549 cells usually have a cobblestone epithelial morphology and corresponding growth pattern. When stimulated with TGF-β1, the cells were similar with fibroblasts, the connections between cells became loose.
USAGE of the Active Transforming Growth Factor Beta 1 (TGFb1)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Transforming Growth Factor Beta 1 (TGFb1)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Transforming Growth Factor Beta 1 (TGFb1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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