Active Taxilin Alpha (TXLNa)

IL14; TXLN; HMW-BCGF; High Molecular Weight B-Cell Growth Factor; Interleukin 14

  • Active Taxilin Alpha (TXLNa) Packages (Simulation)
  • Active Taxilin Alpha (TXLNa) Packages (Simulation)
  • Active Taxilin Alpha (TXLNa) Figure. Gene Sequencing (Extract)
  • APB182Hu01.png Figure. SDS-PAGE
  • Active Taxilin Alpha (TXLNa) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Taxilin Alpha (TXLNa)

Figure. The binding activity of TXLNa with PDIA3.
Taxilin Alpha (TXLNa) also known as interleukin-14 (IL-14) is a cytokine that controls the growth and proliferation of both normal and cancerous B cells. TXLNa induces B-cell proliferation, inhibits antibody secretion, and expands selected B-cell subgroups. This interleukin is produced mainly by T cells and certain malignant B cells. Besides, Protein Disulfide Isomerase A3 (PDIA3) has been identified as an interactor of TXLNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TXLNa and recombinant human PDIA3. Briefly, TXLNa were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PDIA3-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TXLNa pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TXLNa and PDIA3 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active Taxilin Alpha (TXLNa)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Taxilin Alpha (TXLNa)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Taxilin Alpha (TXLNa)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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