Active Stem Cell Factor (SCF)
KITLG; MGF; SF; KL1; Kitl; FPH2; Kit-Ligand; Steel Factor; Mast Cell Growth Factor; Steel factor; Familial Progressive Hyperpigmentation 2
- Product No.APA120Ra61
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point4.9
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 348
For more details, please contact local distributors!US$ 870
For more details, please contact local distributors! US$ 1740
For more details, please contact local distributors! US$ 5220
For more details, please contact local distributors! US$ 13050
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Stem Cell Factor (SCF)
Stem cell factor (SCF), also known as mast cell growth factor (MGF), and steel factor (SLF), plays an important role in hematopoiesis, spermatogenesis and melanogenesis. SCF has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 2 x 104 cells/well and incubated for 72h in the presence or absence of various concentrations of SCF at 37 ℃. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell proliferation of TF-1 cells after incubation with SCF for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of SCF was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells .The ED50 for this effect is typically 2.9 ng/ml.
USAGE of the Active Stem Cell Factor (SCF)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Stem Cell Factor (SCF)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Stem Cell Factor (SCF)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| EPA120Ra61 | Eukaryotic Stem Cell Factor (SCF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA120Ra61 | Active Stem Cell Factor (SCF) | Cell culture; Activity Assays. |
| RPA120Ra01 | Recombinant Stem Cell Factor (SCF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA120Ra01 | Polyclonal Antibody to Stem Cell Factor (SCF) | WB; IHC; ICC; IP. |
| MAA120Ra21 | Monoclonal Antibody to Stem Cell Factor (SCF) | WB; IHC; ICC; IP. |
| SEA120Ra | ELISA Kit for Stem Cell Factor (SCF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA120Ra | Multiplex Assay Kit for Stem Cell Factor (SCF) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |