Active Receptor Tyrosine Protein Kinase erbB-2 (ErbB2)
CD340; EGFR2; HER-2/neu; HER2; NEU; MLN 19; NGL; TKR1; p185; Epidermal Growth Factor Receptor 2; V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2; Neuro/Glioblastoma Derived Oncogene Homolog
- Product No.APB867Hu02
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point6.4
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$
For more details, please contact local distributors!US$
For more details, please contact local distributors! US$
For more details, please contact local distributors! US$
For more details, please contact local distributors! US$
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Receptor Tyrosine Protein Kinase erbB-2 (ErbB2)
Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) as known as HER2 (Human Epidermal Growth Factor Receptor 2), is a member of the EGFR family of receptor tyrosine kinases. It is a 185 kDa cell membrane receptor that plays a crucial role in cell signaling and proliferation.Besides,Plakophilin 2 (PKP2) has been identified as an interactor of ErbB2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human ErbB2 and recombinant human PKP2. Briefly, ErbB2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PKP2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ErbB2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human ErbB2 and recombinant human PKP2 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Receptor Tyrosine Protein Kinase erbB-2 (ErbB2)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Receptor Tyrosine Protein Kinase erbB-2 (ErbB2)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Receptor Tyrosine Protein Kinase erbB-2 (ErbB2)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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