Active Proenkephalin (PENK)
Preproenkephalin; Proenkephalin-A; Synenkephalin; Met-enkephalin; Met-enkephalin-Arg-Gly-Leu; Leu-enkephalin; Met-enkephalin-Arg-Phe
- Product No.UAPD396Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point5.3
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$
For more details, please contact local distributors!US$
For more details, please contact local distributors! US$
For more details, please contact local distributors! US$
For more details, please contact local distributors! US$
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Proenkephalin (PENK)
Proenkephalin (PENK) is a protein encoded by the PENK gene. It is initially synthesized as a preproprotein and undergoes proteolytic processing to generate multiple products. As PENK has the function of inhibit the migration cells，we measure the activity of recombinant mouse PENK by the ability of the protein to inhibit the migration of U2OS cells. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the inhibition of chemotactic effect of recombinant mouse PENK on U2OS cell line. Briefly, U2OS cells were seeded into the upper chambers and PENK was added in lower chamber with a polycarbonate filter (8 μm pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 2h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×10) and the number of migrated cells were counted randomly (five fields for each filter). Result shows MIP-3α is able to induce migration of U2OS cells. The migrated U2OS cells in low chamber at low magnification (×10) were shown in Figure 1.
USAGE of the Active Proenkephalin (PENK)
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
STORAGE of the Active Proenkephalin (PENK)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Proenkephalin (PENK)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| UAPD396Mu01 | Active Proenkephalin (PENK) | Cell culture; Activity Assays. |
| URPD396Mu02 | Recombinant Proenkephalin (PENK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPD396Mu01 | Recombinant Proenkephalin (PENK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAD396Mu01 | Polyclonal Antibody to Proenkephalin (PENK) | WB; IHC; ICC; IP. |
| UPAD396Mu02 | Polyclonal Antibody to Proenkephalin (PENK) | WB; IHC; ICC; IP. |
| USED396Mu | ELISA Kit for Proenkephalin (PENK) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMD396Mu | Multiplex Assay Kit for Proenkephalin (PENK) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |