Active Perforin 1 (PRF1)
FLH2; HPLH2; P1; PFP; Pore Forming Protein; Cytolysin; Lymphocyte pore-forming protein
- Product No.APB317Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point7.4
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
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Packages (Simulation)
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Packages (Simulation)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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Figure. Western Blot
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Perforin 1 (PRF1)
Perforin 1 (PRF1) is a pore forming cytolytic protein found in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin binds to the target cell's plasma membrane, and oligomerises in a Ca2 dependent manner to form pores on the target cell. The pore formed allows for the passive diffusion of a family of pro-apoptotic proteases, known as the granzymes, into the target cell. The activity of recombinant PREF1 was measured by lysis of erythrocytes using a hemolysis assay. A general procedure is as fllows: two-fold dilute the recombinant mouse PREF1 with 0.9% NaCl, add 50μl a serial dilution of PREF1, 10μl 0.1M CaCl2 to each well, then add 50μl 0.25% rabbit erythrocyte (RaE) to each well and mixed gently. Add 50μl 0.9% NaCl to reaplace PREF1 in control wells. The plate is incubated for 20 hours at 37 ℃, 5% CO2. The results are shown in Figure 1. It was obvious that the minimal effective concentration of PREF1 is 2.5μg/ml.
USAGE of the Active Perforin 1 (PRF1)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Perforin 1 (PRF1)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Perforin 1 (PRF1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPB317Mu01 | Recombinant Perforin 1 (PRF1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB317Mu02 | Recombinant Perforin 1 (PRF1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB317Mu01 | Active Perforin 1 (PRF1) | Cell culture; Activity Assays. |
| APB317Mu02 | Active Perforin 1 (PRF1) | Cell culture; Activity Assays. |
| PAB317Mu01 | Polyclonal Antibody to Perforin 1 (PRF1) | WB; IHC; ICC; IP. |
| PAB317Mu02 | Polyclonal Antibody to Perforin 1 (PRF1) | WB; IHC; ICC; IP. |
| LAB317Mu81 | FITC-Linked Polyclonal Antibody to Perforin 1 (PRF1) | WB; IHC; ICC; IF. |
| LAB317Mu71 | Biotin-Linked Polyclonal Antibody to Perforin 1 (PRF1) | WB; IHC; ICC. |
| WEB317Mu | Wide-range ELISA Kit for Perforin 1 (PRF1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEB317Mu | ELISA Kit for Perforin 1 (PRF1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB317Mu | Multiplex Assay Kit for Perforin 1 (PRF1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |