Active Peptidyl Arginine Deiminase Type IV (PADI4)
PAD; PADI5; PDI4; PDI5; Peptidyl Arginine Deiminase Type V; Peptidylarginine deiminase IV
- Product No.APF709Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point6.0
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
-
FOB
US$
For more details, please contact local distributors!US$
For more details, please contact local distributors! US$
For more details, please contact local distributors! US$
For more details, please contact local distributors! US$
For more details, please contact local distributors!
-
Packages (Simulation)
-
Packages (Simulation)
-
Figure. SDS-PAGE
-
ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Peptidyl Arginine Deiminase Type IV (PADI4)
Peptidylarginine deiminase 4 (PADI4) is an enzyme that plays an important role in the organism. It mainly catalyzes the conversion of arginine residues in proteins into citrulline residues. PADI4 plays a key role in various physiological processes such as cell differentiation and immune response. The abnormal expression or change in its activity is closely related to autoimmune diseases like rheumatoid arthritis and systemic lupus erythematosus, making it an important target in the research of these related diseases.The activity of recombinant human PADI4 is determined by its ability to catalyze Benzoyl-arginine ethyl ester (BAEE) to form citrulline.The reaction was performed in 50 mM Tris, 0.1 M NaCl, 10 mM CaCl2, 5 mM DTT solution (Assay Buffer, pH 7.5). A total volume of 300 μL was achieved by adding 75 μL of 100 μg/mL PADI4 to 75 μL of 10 mM BAEE. After 30-minute incubation at 37°C, the reaction mixture was acidified with color-developing solution containing ammonium iron sulfate and measured at 540 nm .
USAGE of the Active Peptidyl Arginine Deiminase Type IV (PADI4)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Peptidyl Arginine Deiminase Type IV (PADI4)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Peptidyl Arginine Deiminase Type IV (PADI4)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
INCREMENT SERVICES
BCA Protein Quantification Kit
Molecular Mass Marker for Protein
Monoclonal Antibody Customized Service
Polyclonal Antibody Customized Service
Protein Activity Test Experiment Service
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
Buffer
Lentivirus Packaging Experiment Service
Adenovirus Packaging Experiment Service
Real Time PCR Experimental Service
Spike RBD Protein (S-RBD)
Protein G
Protein A