Active Noggin (NOG)
SYM1; SYNS1; Synostoses(Multiple)Syndrome 1; Symphalangism 1(Proximal)
- Product No.UAPC130Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point10.0
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 131
For more details, please contact local distributors!US$ 327
For more details, please contact local distributors! US$ 654
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For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Gene sequencing
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SDS-PAGE
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Figure. Western Blot; Sample: Recombinant NOG, Rat.
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Noggin (NOG)
NOG (Noggin) is a signaling molecule that plays an important role in promoting somite patterning in the developing embryo, essential for cartilage morphogenesis and joint formation. It is considered as an inhibitor in bone morphogenetic proteins (BMP) signaling, by binding with BMP4, thus a binding ELISA assay was conducted to detect the association of recombinant rat NOG with BMP4. Briefly, NOG were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP4-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-NOG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of NOG with BMP4 was shown in Figure 1 and this effect was in a dose dependent manner.
USAGE of the Active Noggin (NOG)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Noggin (NOG)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Noggin (NOG)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| URPC130Ra01 | Recombinant Noggin (NOG) | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPC130Ra01 | Active Noggin (NOG) | Cell culture; Activity Assays. |
| UAPC130Ra02 | Active Noggin (NOG) | Cell culture; Activity Assays. |
| URPC130Ra02 | Recombinant Noggin (NOG) | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAC130Ra01 | Polyclonal Antibody to Noggin (NOG) | WB; IHC; ICC; IP. |
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| USEC130Ra | ELISA Kit for Noggin (NOG) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMC130Ra | Multiplex Assay Kit for Noggin (NOG) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| UKSC130Ra01 | ELISA Kit DIY Materials for Noggin (NOG) | Main materials for "Do It (ELISA Kit) Yourself". |