Active Neuraminidase (NEU)
NEU1; SIAL1; Sialidase 1; Lysosomal Sialidase; Acetylneuraminyl hydrolase; G9 sialidase; N-acetyl-alpha-neuraminidase 1
- Product No.APB611Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point5.5
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 320
For more details, please contact local distributors!US$ 800
For more details, please contact local distributors! US$ 1600
For more details, please contact local distributors! US$ 4800
For more details, please contact local distributors! US$ 12000
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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SDS-PAGE
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Figure. Western Blot; Sample: Recombinant NEU, Human.
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Neuraminidase (NEU)
NEU (Sialidase-1) is an enzyme that catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (CTSA). Thus a binding ELISA assay was conducted to detect the interaction of recombinant human NEU and recombinant human CTSA. Briefly, NEU were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CTSA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-NEU pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of NEU and CTSA was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Neuraminidase (NEU)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Neuraminidase (NEU)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Neuraminidase (NEU)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
INCREMENT SERVICES
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| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPB611Hu05 | Recombinant Neuraminidase (NEU) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB611Hu02 | Recombinant Neuraminidase (NEU) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB611Hu01 | Recombinant Neuraminidase (NEU) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB611Hu03 | Recombinant Neuraminidase (NEU) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB611Hu01 | Active Neuraminidase (NEU) | Cell culture; Activity Assays. |
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| RPB611Hu04 | Recombinant Neuraminidase (NEU) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB611Hu01 | Polyclonal Antibody to Neuraminidase (NEU) | WB; IHC; ICC; IP. |
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