Active Nerve Growth Factor (NGF)

NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor

  • Active Nerve Growth Factor (NGF) Packages (Simulation)
  • Active Nerve Growth Factor (NGF) Packages (Simulation)
  • APA105Hu61.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Nerve Growth Factor (NGF)

Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. To test the effect of NGF on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 20,000 cells/well with various concentrations of recombinant human NGF. After incubated for 72 hours, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of TF-1 cells after incubation with NGF for 72 hours observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human NGF for 72 hours. The result was shown in Figure 2. It was obvious that recombinant human NGF significantly increased cell viability of TF-1 cells, the EC50 was 0.38 ng/ml.

Figure 2. Cell proliferation of TF-1 cells after stimulated with recombinant human NGF.

USAGE of the Active Nerve Growth Factor (NGF)

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Nerve Growth Factor (NGF)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Nerve Growth Factor (NGF)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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