Active Matrix Metalloproteinase 9 (MMP9)
GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase
- Product No.APA553Po01
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point4.8
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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Figure. Western Blot
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Matrix Metalloproteinase 9 (MMP9)
Figure. Hydrolysis of gelatin by MMP9.<br/>Mechanism: MMP9 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 92kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of recombinant pig MMP9 (1000ng, 500ng, 100ng, 10ng, 1ng, 0.1ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate- polyacrylamide gel (SDS–PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP9 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP9 protein as negative control, and blood sample as positive control. <br/>Result: Gelatin hydrolysis by recombinant pig MMP9 (10-70kd) was shown in figure 1.
USAGE of the Active Matrix Metalloproteinase 9 (MMP9)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Matrix Metalloproteinase 9 (MMP9)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Matrix Metalloproteinase 9 (MMP9)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Catalog No. | Organism species: Sus scrofa; Porcine (Pig) | Applications (RESEARCH USE ONLY!) |
| APA553Po01 | Active Matrix Metalloproteinase 9 (MMP9) | Cell culture; Activity Assays. |
| RPA553Po01 | Recombinant Matrix Metalloproteinase 9 (MMP9) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA553Po01 | Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9) | WB; IHC; ICC; IP. |
| MAA553Po21 | Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9) | WB; IHC; ICC; IP. |
| SEA553Po | ELISA Kit for Matrix Metalloproteinase 9 (MMP9) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA553Po | Multiplex Assay Kit for Matrix Metalloproteinase 9 (MMP9) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |