Active Macrophage Inflammatory Protein 1 Alpha (MIP1a)
CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein
- Product No.APA092Mu61
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point4.9
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 240
For more details, please contact local distributors!US$ 600
For more details, please contact local distributors! US$ 1200
For more details, please contact local distributors! US$ 3600
For more details, please contact local distributors! US$ 9000
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Macrophage Inflammatory Protein 1 Alpha (MIP1a)
MIP-1a (macrophage inflammatory protein 1-alpha) also known as Chemokine (C-C motief) ligand 3 (CCL3), is a cytokine belonging to the CC chemokine family that is involved in the recruitment and activation of macrophages, monocytes and neutrophils. In this case, chemotaxis assay used 24-well microchemotaxis system was undertaken to evaluate the chemotactic effect of MIP-1a on the human monocytic cell line THP1. Briefly, THP1 cells were seeded into the upper chambers and various concentrations of MIP-1a was added in lower chamber with a polycarbonate filter (8 um pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 2h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted using Image J. The migrated THP1 cells in low chamber at low magnification (×100) was shown in Figure 1. The number of migrated THP-1 cells was shown in Figure 2. The optimum chemotaxis of MIP-1a occurs at 1 ug/ml.
USAGE of the Active Macrophage Inflammatory Protein 1 Alpha (MIP1a)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Macrophage Inflammatory Protein 1 Alpha (MIP1a)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Macrophage Inflammatory Protein 1 Alpha (MIP1a)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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