Active Lemur Tyrosine Kinase 3 (LMTK3)

LMR3; TYKLM3; Serine/threonine-protein kinase LMTK3

  • Active Lemur Tyrosine Kinase 3 (LMTK3) Packages (Simulation)
  • Active Lemur Tyrosine Kinase 3 (LMTK3) Packages (Simulation)
  • APE375Hu01.jpg Figure. SDS-PAGE
  • Active Lemur Tyrosine Kinase 3 (LMTK3) Figure. Western Blot; Sample: Recombinant LMTK3, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Lemur Tyrosine Kinase 3 (LMTK3)

Mechanism: Lemur tyrosine kinase 3 (LMTK3), a member of the receptor tyrosine kinase (RTK) family, is implicated in breast cancer growth and endocrine resistance.It has been reported that LMTK3 promotes cell invasion, motility, and migration of the MCF-7 breast cancer cell line . To test the bioactivity of LMTK3 ,MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well in serum-free standard DMEM after adding to various concentrations of LMTK3 protein. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly，10 µl of CCK-8 solution was added to each well of the plate，then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 °C .
Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown in Figure 1.Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown in Figure 1.

USAGE of the Active Lemur Tyrosine Kinase 3 (LMTK3)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Lemur Tyrosine Kinase 3 (LMTK3)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Lemur Tyrosine Kinase 3 (LMTK3)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPE375Hu01 Recombinant Lemur Tyrosine Kinase 3 (LMTK3) Positive Control; Immunogen; SDS-PAGE; WB.
APE375Hu01 Active Lemur Tyrosine Kinase 3 (LMTK3) Cell culture; Activity Assays.
PAE375Hu01 Polyclonal Antibody to Lemur Tyrosine Kinase 3 (LMTK3) WB; IHC; ICC; IP.
MAE375Hu21 Monoclonal Antibody to Lemur Tyrosine Kinase 3 (LMTK3) WB; IHC; ICC; IP.
SEE375Hu ELISA Kit for Lemur Tyrosine Kinase 3 (LMTK3) Enzyme-linked immunosorbent assay for Antigen Detection.
LME375Hu Multiplex Assay Kit for Lemur Tyrosine Kinase 3 (LMTK3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
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