Active Lecithin Cholesterol Acyltransferase (LCAT)
phosphatidylcholine-sterol O-acyltransferase; Phospholipid-cholesterol acyltransferase
- Product No.UAPJ516Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point5.8
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 156
For more details, please contact local distributors!US$ 390
For more details, please contact local distributors! US$ 780
For more details, please contact local distributors! US$ 2340
For more details, please contact local distributors! US$ 5850
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Lecithin Cholesterol Acyltransferase (LCAT)
LCAT(Phosphatidylcholine-sterol acyltransferase) is an enzyme in the extracellular metabolism of plasma lipoproteins, which converts cholesterol and phosphatidylcholines(lecithins) to cholesteryl esters and lysophosphatidylcholines. It is reported that Apolipoprotein E (apoE) is the main LCAT activator in glia- conditioned media (GCM). Thus, a binding ELISA assay was conducted to detect the association of recombinant human LCAT with recombinant human apoE. Briefly, recombinant human LCAT were diluted serially in PBS with 0.01% BSA(pH 7.4). Duplicate samples of 100ul were then transferred to apoE-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-LCAT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of LCAT with apoE was shown in Figure 1 ,the EC50 for this effect is 0.036ug/mL.
USAGE of the Active Lecithin Cholesterol Acyltransferase (LCAT)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Lecithin Cholesterol Acyltransferase (LCAT)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Lecithin Cholesterol Acyltransferase (LCAT)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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