Active Isocitrate Dehydrogenase 2, mitochondrial (IDH2)
IDH; IDP; ICD-M; IDHM; mNADP-IDH; Isocitrate dehydrogenase [NADP], mitochondrial; Oxalosuccinate decarboxylase
- Product No.APH838Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point8.3
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
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For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Isocitrate Dehydrogenase 2, mitochondrial (IDH2)
Isocitrate Dehydrogenase 2 (IDH2) catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate. There are two subclasses in the IDH family, one of them utilizing NADP as the electron acceptor and the other using NAD . The protein encoded by this gene is the NADP dependent isocitrate dehydrogenase found in the mitochondrial. The activity of recombinant human IDH2 was measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The reaction was performed in 25 mM Tris, 0.5 mM MnCl2, 5 mM DTT, pH 7.5 (Assay Buffer), initiated by addition 50 μL of various concentrations of IDH2 (diluted by Assay Buffer) to 50 µL of substrate mixture consisted of 1 mM NADP and 2 mM isocitric acid. The final well serves as a negative control with no IDH2, replaced with 50μl assay buffer. Incubated at 25℃ for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant human IDH2 is >200 pmol/min/µg.
USAGE of the Active Isocitrate Dehydrogenase 2, mitochondrial (IDH2)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Isocitrate Dehydrogenase 2, mitochondrial (IDH2)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Isocitrate Dehydrogenase 2, mitochondrial (IDH2)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| APH838Hu01 | Active Isocitrate Dehydrogenase 2, mitochondrial (IDH2) | Cell culture; Activity Assays. |
| RPH838Hu01 | Recombinant Isocitrate Dehydrogenase 2, mitochondrial (IDH2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAH838Hu01 | Polyclonal Antibody to Isocitrate Dehydrogenase 2, mitochondrial (IDH2) | WB,IHC |
| MAH838Hu21 | Monoclonal Antibody to Isocitrate Dehydrogenase 2, mitochondrial (IDH2) | WB; IHC; ICC; IP. |
| SEH838Hu | ELISA Kit for Isocitrate Dehydrogenase 2, mitochondrial (IDH2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMH838Hu | Multiplex Assay Kit for Isocitrate Dehydrogenase 2, mitochondrial (IDH2) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |