Active Interleukin 6 (IL6)
MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor
- Product No.APA079Ra61
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer FormulationPBS, pH7.6, containing 5% trehalose.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point7.8
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 280
For more details, please contact local distributors!US$ 700
For more details, please contact local distributors! US$ 1400
For more details, please contact local distributors! US$ 4200
For more details, please contact local distributors! US$ 10500
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Gene sequencing
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SDS-PAGE
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Figure. Western Blot; Sample: Recombinant IL6, Rat.
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Interleukin 6 (IL6)
Interleukin-6 (IL-6), a pro-inflammatory cytokine and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL-6 significantly increased the MMP-10 protein lever in the human lung cancer A549 cells through the JAK/STAT signaling pathway. Briefly, A549 cells were seeded into 6-well cell culture clusters and allowed to grow to 50-70% confluence, then different concentrations of IL-6 was added. After incubated for 24h, the protein levels of MMP-10 in the cell supernatant were determined by Western blot.
Result: MMP-10 protein lever significantly increased in A549 cells due to the stimulation of IL-6, the data was shown in Figure 1.
USAGE of the Active Interleukin 6 (IL6)
Reconstitute in PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Interleukin 6 (IL6)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Interleukin 6 (IL6)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
INCREMENT SERVICES
BCA Protein Quantification Kit
Molecular Mass Marker for Protein
Monoclonal Antibody Customized Service
Polyclonal Antibody Customized Service
Protein Activity Test Experiment Service
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
Buffer
Lentivirus Packaging Experiment Service
Adenovirus Packaging Experiment Service
Real Time PCR Experimental Service
Spike RBD Protein (S-RBD)
Protein G
Protein A
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