Active Interleukin 5 (IL5)

E-CSF; ESGF; EDF; TRF; Colony-Stimulating Factor,Eosinophil; T-Cell replacing Factor; B Cell Differentiation Factor I; Eosinophil Differentiation Factor

  • Active Interleukin 5 (IL5) Packages (Simulation)
  • Active Interleukin 5 (IL5) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Interleukin 5 (IL5)

Interleukin-5 (IL-5), a secreted glycoprotein, is a member of the hematopoietic family. Interleukin 5 has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 1x104cells/well and incubated for 48h in the presence or absence of various concentrations of IL-5 at 37oC. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC.
Cell proliferation of TF-1 cells after incubation with IL-5 for 48h observed by inverted microscope was shown in Figure 1.

The dose-effect curve of IL-5 was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The ED50 for this effect is typically 0.18 to 7.675ng/mL.

USAGE of the Active Interleukin 5 (IL5)

Reconstitute in ddH2O to a concentration of 0.1-0.2 mg/mL. Do not vortex.

STORAGE of the Active Interleukin 5 (IL5)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Interleukin 5 (IL5)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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