Active Interleukin 4 (IL4)
BSF1; BCGF1 ;B Cell Stimulatory Factor 1; Lymphocyte stimulatory factor 1; B Cell Growth Factor; Binetrakin; Pitrakinra
- Product No.UAPA077Ov01
- Organism SpeciesOvis aries; Ovine (Sheep) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point9.4
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$
For more details, please contact local distributors!US$
For more details, please contact local distributors! US$
For more details, please contact local distributors! US$
For more details, please contact local distributors! US$
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Interleukin 4 (IL4)
Interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells.It plays vital biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells and monocyte. It is a key regulator in humoral and adaptive immunity. IL4 induces B-cell class switching to IgE, and up-regulates MHC class II production. To test the effect of sheep IL4 on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant sheep IL4. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant sheep IL4 for 72h. The ED50 of recombinant sheep IL4 is 0.077 μg/ml.
USAGE of the Active Interleukin 4 (IL4)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Interleukin 4 (IL4)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Interleukin 4 (IL4)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
INCREMENT SERVICES
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Molecular Mass Marker for Protein
Monoclonal Antibody Customized Service
Polyclonal Antibody Customized Service
Protein Activity Test Experiment Service
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
Buffer
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Spike RBD Protein (S-RBD)
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Related products
| Catalog No. | Organism species: Ovis aries; Ovine (Sheep) | Applications (RESEARCH USE ONLY!) |
| UAPA077Ov01 | Active Interleukin 4 (IL4) | Cell culture; Activity Assays. |
| URPA077Ov01 | Recombinant Interleukin 4 (IL4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA077Ov01 | Polyclonal Antibody to Interleukin 4 (IL4) | WB; IHC; ICC; IP. |
| UMAA077Ov21 | Monoclonal Antibody to Interleukin 4 (IL4) | WB; IHC; ICC; IP. |
| USEA077Ov | ELISA Kit for Interleukin 4 (IL4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA077Ov | Multiplex Assay Kit for Interleukin 4 (IL4) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |