Active Interleukin 34 (IL34)
C16orf77
- Product No.UAPC007Hu63
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point6.0
- ApplicationsCell culture; Activity Assays.
- Download n/a
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 159
For more details, please contact local distributors!US$ 398
For more details, please contact local distributors! US$ 795
For more details, please contact local distributors! US$ 2385
For more details, please contact local distributors! US$ 5963
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Interleukin 34 (IL34)
Interleukin 34 (IL-34) is a protein belonging to a group of cytokines called interleukins. IL-34 increases growth or survival of immune cells known as monocytes; it elicits its activity by binding the Colony stimulating factor 1 receptor. To test the effect of IL-34 on cell proliferation, TE-1 cells were seeded into triplicate wells of 96-well plates which contains various concentrations of recombinant human IL-34. After incubated for 3 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37 ℃. Cell viability was assessed by CCK-8(Cell Counting Kit-8 ) assay. The result was shown in Figure 1. It was obvious that IL-34 significantly increased cell viability of TE-1 cells. The ED50 is 2.252 µg/mL.
USAGE of the Active Interleukin 34 (IL34)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Interleukin 34 (IL34)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Interleukin 34 (IL34)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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