Active Interleukin 11 (IL11)
AGIF; Adipogenesis Inhibitory Factor; Oprelvekin
- Product No.UAPA057Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point11.7
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
-
FOB
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ACTIVITY TEST of the Active Interleukin 11 (IL11)
Interleukin 11 (IL-11) is a multifunctional cytokine. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL-11 has been demonstrated to improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses and participate in the regulation of bone cell proliferation and differentiation. Besides, IL-11 has been proved to promote cell proliferation of TF-1 cells. 2x104 TF-1 cells were seeded into a well of 96-well plates and allowed to attach, replaced with various concentrations of recombinant human TRAIL. After incubated for 72 hours, the 96-well plate was read at OD450 nm. The result was shown in Figure1. It was obvious that recombinant IL-11 protein significantly increased cell viability of TF-1 cells. Its EC50 is 0.5677μg/mL.
USAGE of the Active Interleukin 11 (IL11)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Interleukin 11 (IL11)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Interleukin 11 (IL11)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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