Active Interleukin 1 Receptor Antagonist (IL1RA)
IL1RN; IL1-RA; ICIL1RA; IL1ra3; IL1F3; IRAP;IL-1RA; Interleukin-1 Family Member 3; IL1 inhibitor; Anakinra
- Product No.APA223Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point5.4
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 216
For more details, please contact local distributors!US$ 540
For more details, please contact local distributors! US$ 1080
For more details, please contact local distributors! US$ 3240
For more details, please contact local distributors! US$ 8100
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Figure. Gene Sequencing (extract)
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Figure. SDS-PAGE
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Sample: Recombinant IL1RA, Human;
Antibody: Rabbit Anti-Human IL1RA Ab (PAA223Hu06)
Figure. Western Blot
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Interleukin 1 Receptor Antagonist (IL1RA)
IL-1RA is an agent that binds non-productively to the cell surface interleukin-1 receptor (IL-1R), the same receptor that binds interleukin 1 (IL-1), preventing IL-1 from sending a signal to that cell. Besides, Interleukin 1 Alpha (IL-1a) has been identified as an interactor of IL-1RA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL-1RA and recombinant human IL-1a. Briefly, IL1RA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to IL-1a-coated microtiter wells and incubated for 2 h at 37℃. Wells were washed with PBST and incubated for 1 h with anti-IL-1RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL-1RA and IL-1a was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of IL-1RA with IL-1a.
USAGE of the Active Interleukin 1 Receptor Antagonist (IL1RA)
Reconstitute in 10mM PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Interleukin 1 Receptor Antagonist (IL1RA)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Interleukin 1 Receptor Antagonist (IL1RA)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
INCREMENT SERVICES
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Molecular Mass Marker for Protein
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