Active Interleukin 1 Beta (IL1b)
IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin
- Product No.APA563Fi01
- Organism SpeciesDanio rerio (Zebrafish) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point5.4
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
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Packages (Simulation)
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Packages (Simulation)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Interleukin 1 Beta (IL1b)
Interleukin 1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. It has been reported that IL-1β can induced IL-8 production in A549 cells. To test the bioactivity of recombinant zebrafish IL-1β, A549 cells were seeded into 24-well plate at a density of 1x105 cells/mL , and allowed to attach overnight before treated with certain concentrations of recombinant zebrafish IL-1β for 48h and IL-8 levels in the cell supernatant were determined by ELISA (SEA080Hu). IL-8 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL-1β which was shown in Figure1, the EC50 was 16.86 ug/ml.
USAGE of the Active Interleukin 1 Beta (IL1b)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Interleukin 1 Beta (IL1b)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Interleukin 1 Beta (IL1b)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Related products
| Catalog No. | Organism species: Danio rerio (Zebrafish) | Applications (RESEARCH USE ONLY!) |
| APA563Fi01 | Active Interleukin 1 Beta (IL1b) | Cell culture; Activity Assays. |
| RPA563Fi01 | Recombinant Interleukin 1 Beta (IL1b) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA563Fi01 | Polyclonal Antibody to Interleukin 1 Beta (IL1b) | WB; IHC; ICC; IP. |