Active Interferon Gamma (IFNg)
IFN-G; IFG; IFI; INFr; IFN, Immune Interferon
- Product No.UAPA049Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point8.8
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
US$ 104
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For more details, please contact local distributors! US$ 3900
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Gene sequencing
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Figure. SDS-PAGE
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WB Image
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ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Interferon Gamma (IFNg)
IFN-γ is an important activator of macrophages, it promotes production of inducible Nitric Oxide Synthase (iNOS) in macrophages. After stimulated with IFN-γ, morphological changes will occur in murine macrophage cell line (Raw 246.7 cells), and inducible nitric-oxide synthase (iNOS) in the cells will increase. Raw 246.7 cells were incubated in DMEM with IFN-γ (2ng/mL) for 24h, then cells were observed by inverted microscope and iNOS in cell lysates was detected by ELISA.
USAGE of the Active Interferon Gamma (IFNg)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Interferon Gamma (IFNg)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Interferon Gamma (IFNg)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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